Morphofunctional state of erythrocytes in benign obstructive jaundice

Abstract

OBJECTIVE. Structural and functional properties of erythrocytes were researched in patients with benign obstructive jaundice of different degree of severity. MATERIAL AND METHODS. Patients (n =81) with obstructive jaundice of benign genesis were examined at the period from 2013 till 2016. The control group consisted of 29 healthy patients. The atomic-force microscopy of erythrocytes was applied in all patients in order to study structural and functional properties of erythrocytes. RESULTS. There was noted, that growth of bilirubin conсentration caused the increase of erythrocyte size. Binocave red blood cells acquired horizontal orientation in patients with B class of obstructive jaundice. Erythrocytes transformed to domed stomatocyte and their square increased, volume of red cells become twice more compared with normal state in patients with C class of obstructive jaundice. CONCLUSIONS. The degree of severity of obstructive jaundice caused red cell transformation. The binocave erythrocytes changed to the domed stomatocytes. This transformation significantly increased erythrocyte volume, the number of ruptures and their volume on bilipidic membrane layer.